Vitamin B-9 and MTHFR variants


Do common variations in the MTHFR gene keep vitamin B from working? Can a group of ordinary people come together to find out? And maybe find a treatment?


In the MTHFR gene (methylenetetrahydrofolate reductase), 2 small variations in DNA (SNPs rs1801133/C677T & rs1801131/A1298C) keep vitamin B9 (or folic acid) from being metabolized into its active form (folate).

Without this form of vitamin B, homocysteine may accumulate. This may lead to nutritional deficiencies and symptoms associated with diabetes complications, including vascular damage and nerve damage. This is also associated with blood clots and pregnancy loss.

Up to 60% of people may have some form of MTHFR mutation.

We aim to:
  • Find people with MTHFR mutations - by collecting genotype data from volunteers who have used genetic testing services.
  • Try simple interventions - like special vitamin B supplements available over-the-counter.
  • See if they work - by asking participants to share results from blood tests performed at commercial labs.
Drug companies won't do this type of study -- there's little money to be made in over-the-counter treatments.

But we can. The tools to do this -- to look at genomic information, to measure treatment results, and to analyze the data -- are now cheap or free.

All we need are concerned people who care enough to help. Want to answer this question? Want to help build community and tools that let people take personal charge of their health?

Inclusion Criteria

Please no participants with known vitamin B deficiences, anyone not able to follow the outlined vitamin protocol, or anyone who might have health problems related to high homocysteine levels.

Day 1: Submit your personal genomic data on the specified MTHFR variants (handled on joining study)

Day 1: No Vitamin B supplements for 1 week

To establish a washout baseline, take no supplements containing vitamin B for a week.
Repeat every day, ending after 7 occurrences.

Day 8: Homocysteine Blood Test

Obtain a test for serum homocysteine in the blood.



  • Check out the FAQ / informed consent (in development), and sign off on it.
  • Submit data on MTHFR variants.
  • Participate in at least 3 different 2 week trials, involving taking over-the-counter vitamins, then measuring homocysteine levels with a blood draw each time (blood tests cost about $70 each).
  • Record your blood test data here



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Does anyone have experience with vitamin B complex sub cutaneous shots verses IM? My Dr. Prescribed them & my research shows that b vitamins can be absorbed subcutaneously and are longer acting than IM?

posted by MelodyGallka

MelodyGallka I am homozygous for C677T diagnosed 3/2015. I am 55 & have been searching for what s wrong with me & my mom since my 20's. . No doctors could figure it out. While she lived a very long life it was miserable & filled with mental illness & pain. My dad tried to help her with vitamins. She always had a sore tongue Despite taking B supplements. Now she is gone & it is my turn. No doctors have knowledge about this disorder & treat you like you are crazy or prescribe off the cuff without much precision. I changed my diet first to heal my gut. Then I added methylated folate slowly 1 up to 5 mg. per day. I began having a sore tongue about 1 week into the treatment. So I added 2500 mcg. Of mytyated B12 2x/day. I continued this protocol since 5/2015 with some relief of symptoms & some increase: tingling & clumsiness of the hands feet, brain fog & memory issues, fatigue, now sore tongue) 6/30 I added vit b complex shots .5 compounded subcutaneously. I tested my blood for b12/folate serum before. See what happens.


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There has been a lot of buzz on some of the Facebook support groups (LDN, Fibromyalgia, ME/CFS, MS, Lyme, etc.) about the fact that so many people are testing positive for MTHFR and also having these diseases. Would there be any chance of asking this question to the participants? I heard of one study for chronic Lyme that out of 200 subjects 198 had the mutation.

posted by lisaacso

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gotnospice Not sure if I should join, what genotypes for rs1801133 and 31 should I have to be able to join, or to have to worry?


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